PEPTIDE RECONSTITUTION GUIDE

A Beginner’s Guide to Preparing Lyophilised Research Materials

What Is Reconstitution?

Reconstitution is the process of adding a sterile diluent (commonly bacteriostatic water) to a lyophilised (freeze-dried) research material to return it to liquid form for laboratory handling and measurement.

Equipment Required

✅ Lyophilised research vial
✅ Bacteriostatic water
✅ Sterile syringe
✅ Alcohol swabs
✅ Clean workspace

Step 1: Prepare Your Workspace

• Wash hands thoroughly.
• Clean the vial stoppers with alcohol swabs.
• Allow surfaces to dry completely.

Step 2: Draw the Diluent

• Using a sterile syringe, draw the required volume of bacteriostatic water.
• Check for air bubbles and remove if necessary.

Step 3: Add the Diluent

• Insert the needle through the vial stopper.
• Slowly allow the water to run down the inside wall of the vial.
• Avoid spraying directly onto the powder.

Step 4: Dissolve the Material

• Gently swirl the vial.
• Do not shake vigorously.
• Allow the powder to dissolve completely.

Step 5: Label & Store

Record:

• Product name
• Date reconstituted
• Amount of diluent added

Store according to manufacturer recommendations, typically refrigerated between 2–8°C.